RT-PCR

RT-PCR is a pretty standard procedure, and now its use is extremely broad. At its most basic application, PCR can be extended to small amount of template DNA (or RNA) in large quantities within a few hours. This is done by mixing the DNA on both sides of the DNA primers (reverse), Taq polymerase (Thermus aquaticus type, polymerase thermophile that is able to withstand very high temperatures), free nucleotides (dNTPs on DNA, RNA, NTPs), and buffer. The temperature is then denatured, and alternating hot and cold reanneal DNA polymerase, the addition of new complementary strands each time. In addition to the main use of PCR, which is specially designed primers will ligate to two different pieces of DNA together, or add the restriction site, and many other creative applications. It is clear that PCR is a procedure that is an integral addition to molecular biology tools and techniques are constantly improved over the years.

Real-Time PCR machine

The measurement of the pros and cons of different real-time PCR, or qPCR platforms for its own laboratory, factors to consider are the following: support of chemical processes, multiple personality, chemistry, productivity, flexibility, size, ease of use and powerful software package, reproducibility, speed, size, technical assistance, support, and not the costs, not just the initial cost of equipment and services, as well as the related costs of consumables and reagents. It is also possible to "try before you buy", most companies offer a loan machine. This makes sense in some of them, if you narrowed down your choices. The user experience can not be ignored, and now there are several useful web sites and groups where the e-mail questions and inquiries.